| 000 | 07174cam a2201033 a 4500 | ||
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| 001 | ocn827017835 | ||
| 003 | OCoLC | ||
| 005 | 20250702165820.0 | ||
| 006 | m o d | ||
| 007 | cr ||||||||||| | ||
| 008 | 130208s2013 nyua ob 001 0 eng d | ||
| 040 |
_aSCB _beng _epn _cSCB _dSCB _dGW5XE _dCOO _dOHS _dYDXCP _dUBY _dOCLCA _dOCLCF _dEUW _dOCLCQ _dOCLCO _dOCLCQ _dVT2 _dLVT _dOTZ _dTFH _dOCLCO _dOCLCA _dBUF _dKIJ _dOCLCO _dOCLCQ _dOCLCO _dCASUM _dMERER _dOCLCQ _dU3W _dOCLCO _dOCLCQ _dOCLCO _dAU@ _dOCLCO _dOCLCQ _dYOU _dOCLCO _dOCLCQ _dOCLCO _dOCLCQ _dWURST _dOCLCA _dOCLCO _dOCLCQ _dOCLCO _dOCLCL _dOCLCQ |
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| 019 |
_a964890194 _a967627731 _a985035108 _a1005766774 _a1027485458 _a1058210731 _a1081189871 |
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| 020 |
_a9781627032780 _q(electronic bk.) |
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| 020 |
_a1627032789 _q(electronic bk.) |
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| 020 | _a1627032770 | ||
| 020 | _a9781627032773 | ||
| 020 | _z9781627032773 | ||
| 024 | 7 |
_a10.1007/978-1-62703-278-0 _2doi |
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| 029 | 1 |
_aAU@ _b000061122467 |
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| 029 | 1 |
_aCHBIS _b007619553 |
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_aCHVBK _b185424643 |
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_aNZ1 _b15212736 |
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| 035 |
_a(OCoLC)827017835 _z(OCoLC)964890194 _z(OCoLC)967627731 _z(OCoLC)985035108 _z(OCoLC)1005766774 _z(OCoLC)1027485458 _z(OCoLC)1058210731 _z(OCoLC)1081189871 |
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| 050 | 4 | _aQK981.4 | |
| 060 | 4 |
_aW1 _bME9616J v.975 2013 |
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| 060 | 4 | _aQK 981.4 | |
| 072 | 7 |
_aMED107000 _2bisacsh |
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| 072 | 7 |
_aMFN _2bicssc |
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| 082 | 0 | 4 |
_a572.8/652 _223 |
| 049 | _aMAIN | ||
| 245 | 0 | 0 |
_aVirus-induced gene silencing : _bmethods and protocols / _cedited by Annette Becker. |
| 260 |
_aNew York : _bHumana Press, _c2013. |
||
| 300 |
_a1 online resource (xi, 221 pages) : _billustrations (some color) |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 347 |
_atext file _bPDF _2rda |
||
| 490 | 1 |
_aMethods in molecular biology, _x1940-6029 ; _vv. 975 |
|
| 504 | _aIncludes bibliographical references and index. | ||
| 505 | 0 | 0 |
_tVirus-induced gene silencing (VIGS) in plants: An overview of target species and the virus-derived vector systems -- _tRationale for developing new virus vectors to analyze gene function in grasses through virus-induced gene silencing -- _tVirus-induced gene silencing for rice using agroinoculation -- _tUtilizing virus-induced gene silencing for the functional characterization of maize genes during infection with the fungal pathogen ustilago maydis -- _tAnalysis of developmental control genes using virus-induced gene silencing -- _tVirus-induced gene silencing in the rapid cycling columbine aquilegia coerulea "Origami" -- _tVirus-induced gene silencing of the alkaloid-producing basal eudicot model plant eschscholzia californica (California poppy) -- _tVirus-induced gene silencing using artificial miRNAs in nicotiana benthamiana -- _tThe use of vigs technology to study plant-herbivore interactions -- _tVirus-aided gene expression and silencing using TRV for functional analysis of floral scent-related genes -- _tVirus-induced gene silencing in soybean and common bean -- _tFunctional genomic analysis of cotton genes with agrobacterium-mediated virus-induced gene silencing -- _tHighly efficient virus-induced gene silencing in apple and soybean by apple latent spherical virus vector and biolistic inoculation -- _tVIGS: A tool to study fruit development in Solanum lycopersicum -- _tA protocol for VIGS in arabidopsis thaliana using a one-step TYMV-derived vector -- _tVirus-induced gene silencing in strawberry fruit. |
| 588 | 0 | _aPrint version record. | |
| 520 | _aPlants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant's development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions. | ||
| 546 | _aEnglish. | ||
| 650 | 0 |
_aPlant gene silencing. _988594 |
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| 650 | 0 |
_aGene expression. _922751 |
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| 650 | 2 |
_aGene Expression Regulation, Plant _980826 |
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| 650 | 2 | _aGene Silencing | |
| 650 | 2 |
_aGene Expression _922751 |
|
| 650 | 6 |
_aPlantes _xInactivation génique. _9970711 |
|
| 650 | 6 |
_aExpression génique. _9101514 |
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| 650 | 7 |
_aGene expression _2fast _922751 |
|
| 650 | 7 |
_aPlant gene silencing _2fast _988594 |
|
| 650 | 7 |
_aBiology. _2hilcc _94937 |
|
| 650 | 7 |
_aBotany. _2hilcc _986393 |
|
| 650 | 7 |
_aMedicine. _2hilcc _92865 |
|
| 650 | 7 |
_aHealth & Biological Sciences. _2hilcc _924083 |
|
| 650 | 7 |
_aEarth & Environmental Sciences. _2hilcc _924972 |
|
| 650 | 7 |
_aGenetics. _2hilcc _92539 |
|
| 650 | 7 |
_aPlant Ecology. _2hilcc _987123 |
|
| 650 | 7 |
_aPathology. _2hilcc _980598 |
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| 653 | 0 | 0 | _aplanten |
| 653 | 0 | 0 | _aplants |
| 653 | 0 | 0 | _agenexpressie |
| 653 | 0 | 0 | _agene expression |
| 653 | 0 | 0 | _auitschakelen van genexpressie |
| 653 | 0 | 0 | _agene silencing |
| 653 | 0 | 0 | _aplantenziekteverwekkers |
| 653 | 0 | 0 | _aplant pathogens |
| 653 | 0 | 0 | _afenotypen |
| 653 | 0 | 0 | _aphenotypes |
| 653 | 0 | 0 | _adna sequencing |
| 653 | 0 | 0 | _alaboratoriummethoden |
| 653 | 0 | 0 | _alaboratory methods |
| 653 | 0 | 0 | _aprotocollen |
| 653 | 0 | 0 | _aprotocols |
| 653 | 0 | 0 | _abioinformatics |
| 653 | 1 | 0 | _aBiological Techniques |
| 653 | 2 | 0 | _aPlant Biotechnology |
| 653 | 1 | 0 | _aBiologische technieken |
| 653 | 2 | 0 | _aPlantenbiotechnologie |
| 700 | 1 |
_aBecker, Annette, _d1971- _1https://id.oclc.org/worldcat/entity/E39PCjJJ7Vx7cH8dbjHQwMbdpP _988596 |
|
| 776 | 0 | 8 |
_iPrint version: _tVirus-induced gene silencing. _d[S.l.] : Humana, 2013 _z1627032770 _w(OCoLC)810318084 |
| 830 | 0 |
_aMethods in molecular biology (Clifton, N.J.) ; _vv. 975. _x1064-3745 |
|
| 856 | 4 | 0 | _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-62703-278-0 |
| 938 |
_aYBP Library Services _bYANK _n10258738 |
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_a92 _bATIST |
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_c652742 _d652742 |
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