000			11309cam a2201201 i 4500
001			ocn864681043
003			OCoLC
005			20250702170035.0
006			m o d
007			cr cnu|||unuuu
008			131204s2014 nyua ob 001 0 eng c
010			_a 2013949423
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066			_c(S
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019			_a964898805 _a967618986 _a1005782548 _a1058175619 _a1081182101 _a1228566872
020			_a9781627036702 _q(electronic bk.)
020			_a1627036709 _q(electronic bk.)
020			_a1627036695
020			_a9781627036696
020			_z9781627036696 _q(print)
024	7		_a10.1007/978-1-62703-670-2 _2doi
029	1		_aNZ1 _b15340032
029	1		_aAU@ _b000061121137
035			_a(OCoLC)864681043 _z(OCoLC)964898805 _z(OCoLC)967618986 _z(OCoLC)1005782548 _z(OCoLC)1058175619 _z(OCoLC)1081182101 _z(OCoLC)1228566872
042			_apcc
050		4	_aQR414.5 _b.H858 2014
060		4	_aQW 25
060		4	_aW1 _bME9616J v.1087 2014
072		7	_aMJCM _2bicssc
072		7	_aMED044000 _2bisacsh
082	0	4	_a579.2/569 _223
049			_aMAIN
245	0	0	_aHuman retroviruses : _bmethods and protocols / _cedited by Elisa Vicenzi and Guido Poli, Division of Immunology, Transplant, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy.
264		1	_aNew York : _bHumana Press, _c[2014]
264		4	_c©2014
300			_a1 online resource (xviii, 345 pages) : _billustrations (some color)
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aMethods in Molecular Biology, _x1940-6029 ; _vVolume No. 1087
504			_aIncludes bibliographical references and index.
505	0	0	_gPart I. _tMapping the HIV Life Cycle -- _tVirion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay / _rClaudia Cicala and James Arthos -- _tCryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5[alpha] / _rGongpu Zhao and Peijun Zhang -- _tThe Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating / _rYang Yang, Jeremy Luban, and Felipe Diaz-Griffero -- _tThe Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells / _rAmy E. Hulme and Thomas J. Hope -- _tImaging HIV-1 Nuclear Pre-integration Complexes / _rAnna Cereseto and Mauro Giacca -- _tHIV-1 Reverse Transcription / _rAndrea Cimarelli and Jean-Luc Darlix -- _tRNase H: Specificity, Mechanisms of Action, and Antiviral Target / _rKarin Moelling, Felix Broecker, and John E. Kerrigan -- _tHIV-1 Chromatin, Transcription, and the Regulatory Protein Tat / _rLaurence Colin, Eric Verdin, and Carine Van Lint -- _tHIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export / _rAlan Cochrane -- _tHIV-1 Accessory Proteins: Nef / _rAnke Heigele, Daniel Sauter, Jan Münch, and Frank Kirchhoff -- _tHIV-1 Accessory Proteins: VpR / _rRichard Y. Zhao and Michael I. Bukrinsky -- _tHIV-1 Accessory Proteins: Vpu and Vif / _rAmy Andrew and Klaus Strebel -- _tSIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells / _rGregory Berger and Andrea Cimarelli -- _tImaging of HIV Assembly and Release / _rBarbara Müller and Jacomine Krijnse-Locker.
505	8	0	_gPart II. _tIsolation, Co-Receptor Use, and Cell Tropism of HIV-1 -- _tHIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells / _rStefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti -- _tDetermination of HIV-1 Co-receptor Usage / _rMariangela Cavarelli and Gabriella Scarlatti -- _tThe Macrophage and HIV: Basic Concepts and Methodologies / _rSuzanne Gartner -- _tHIV Infection of Dendritic Cells / _rNajla Nasr, Andrew Harman, Stuart Turville, and Anthony L. Cunningham -- _tHistocultures (Tissue Explants) in Human Retrovirology / _rAnush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis.
505	8	0	_gPart III. _tIn Vivo Quantification of HIV-1 -- _tSingle-Copy Quantification of HIV-1 in Clinical Samples / _rAnn Wiegand and Frank Maldarelli -- _tQuantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells / _rChristine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël.
505	8	0	_gPart IV. _tBiotechnological Aspects of HIV-1 -- _tHIV-1-Based Lentiviral Vectors / _rYing Poi Liu and Ben Berkhout -- _tQuantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone / _rZachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang.
505	8	0	_gPart V. _tHTLVS -- _tInvestigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques / _rGreta Forlani, Roberto S. Accolla, and Giovanna Tosi -- _tProviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR / _rClaudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni -- _tQuantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real-Time RT-PCR (qRT-PCR) / _rIlaria Cavallari, Francesca Rende, and Vincenzo Ciminale.
520			_a"Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore assay and function of accessory genes, largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field."--Publisher's description.
588	0		_aOnline resource; title from PDF title page (Springer Protocols, viewed January 30, 2014).
546			_aEnglish.
650		0	_aRetroviruses _vLaboratory manuals. _982213
650		0	_aHIV (Viruses) _vLaboratory manuals. _984532
650		0	_aHTLV (Viruses) _vLaboratory manuals. _989231
650		0	_aRNA viruses. _92531
650		0	_aOncogenic viruses. _989235
650		0	_aLentiviruses. _982860
650		0	_aViruses. _980666
650		0	_aHIV (Viruses) _984537
650		2	_aLentiviruses, Primate _989234
650		2	_aRNA Viruses _92531
650		2	_aOncogenic Viruses _989235
650		2	_aPublication Formats _915957
650		2	_aVertebrate Viruses _984100
650		2	_aLentivirus _989236
650		2	_aViruses _980666
650		2	_aPublication Characteristics _915959
650		2	_aOrganisms _981486
650		2	_aHIV _989237
650		2	_aRetroviridae _989238
650		2	_aLaboratory Manuals _981487
650		4	_aBiology. _94937
650		4	_aHealth & Biological Sciences. _924083
650		4	_aMicrobiology & Immunology.
650		6	_aRétrovirus _vManuels de laboratoire. _982216
650		6	_aVirus de l'immunodéficience humaine _vManuels de laboratoire. _9970325
650		6	_aDeltarétrovirus _vManuels de laboratoire. _9970773
650		6	_aLentivirus de primates. _9970774
650		6	_aVirus à ARN. _9148659
650		6	_aVirus oncogènes. _9145636
650		6	_aLentivirus. _989236
650		6	_aVirus. _9134875
650		6	_aVirus de l'immunodéficience humaine. _9128906
650		7	_aViruses _2fast _980666
650		7	_aRNA viruses _2fast _92531
650		7	_aOncogenic viruses _2fast _989235
650		7	_aLentiviruses _2fast _982860
650		7	_aHIV (Viruses) _2fast _984537
650		7	_aHTLV (Viruses) _2fast _989232
650		7	_aRetroviruses _2fast _982217
655		7	_aLaboratory manuals _2fast
700	1		_aVicenzi, Elisa, _eeditor _4edc _989240
700	1		_aPoli, Guido, _eeditor _4edc _989241
758			_ihas work: _aHuman retroviruses (Text) _1https://id.oclc.org/worldcat/entity/E39PD3d9GbrpdbjTkfHQVK8JrC _4https://id.oclc.org/worldcat/ontology/hasWork
776	0	8	_iPrint version: _tHuman retroviruses. _dNew York : Humana Press, [2014] _z1627036695 _w(DLC) 2013949423 _w(OCoLC)855905421
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 1087. _x1064-3745
856	4	0	_uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-62703-670-2
880	0		_6505-00/(S _aVirion Attachment & Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand Binding Assay -- CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α -- The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating -- The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells -- Imaging HIV-1 Nuclear Pre-Integration Complexes -- HIV-1 Reverse Transcription -- RNase H: Specificity, Mechanisms of Action, and Antiviral Target -- HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat -- HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export -- HIV-1 Accessory Proteins: Nef -- HIV-1 Accessory Proteins: VpR -- HIV-1 Accessory Proteins: Vpu and Vif -- SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells -- Imaging of HIV Assembly and Release -- HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells -- Determination of HIV-1 Co-Receptor Usage -- The Macrophage and HIV: Basic Concepts and Methodologies -- HIV Infection of Dendritic Cells -- Histocultures (Tissue Explants) in Human Retrovirology -- Single Copy Quantification of HIV-1 in Clinical Samples -- Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells -- HIV-1 Based Lentiviral Vectors -- Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone -- Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques -- Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR -- Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real Time RT-PCR (qRT-PCR).
938			_aYBP Library Services _bYANK _n11322172
938			_aAskews and Holts Library Services _bASKH _nAH40390884
994			_a92 _bATIST
999			_c652864 _d652864