| 000 | 06718cam a2200817 i 4500 | ||
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| 001 | ocn877823584 | ||
| 003 | OCoLC | ||
| 005 | 20250702170146.0 | ||
| 006 | m o d | ||
| 007 | cr cnu|||unuuu | ||
| 008 | 140421t20142014nyua ob 001 0 eng c | ||
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_a9781493903481 _q(electronic bk.) |
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_a1493903489 _q(electronic bk.) |
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| 020 | _a1493903470 | ||
| 020 | _a9781493903474 | ||
| 020 | _z9781493903474 | ||
| 024 | 7 |
_a10.1007/978-1-4939-0348-1 _2doi |
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_aNZ1 _b15574284 |
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| 035 |
_a(OCoLC)877823584 _z(OCoLC)964855297 _z(OCoLC)967620957 _z(OCoLC)1005754636 _z(OCoLC)1229624946 _z(OCoLC)1485300976 |
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| 042 | _apcc | ||
| 050 | 4 | _aRC137 | |
| 060 | 4 |
_aW1 _bME9616J v.1138 2014 |
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| 060 | 4 | _aQW 25 | |
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_aMJCJ _2bicssc |
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_a616.9/1852 _223 |
| 049 | _aMAIN | ||
| 245 | 0 | 0 |
_aDengue : _bmethods and protocols / _cedited by Radhakrishnan Padmanabhan, Subhash G. Vasudevan. |
| 264 | 1 |
_aNew York, NY : _bHumana Press, _c[2014] |
|
| 264 | 4 | _c©2014 | |
| 300 |
_a1 online resource (xiii, 403 pages) : _billustrations (some color) |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 490 | 1 |
_aMethods in Molecular Biology, _x1940-6029 ; _v1138 |
|
| 504 | _aIncludes bibliographical references and index. | ||
| 505 | 0 | 0 |
_tDengue Virus Growth, Purification and Fluorescent Labeling -- _tIsolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique -- _tMeasuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique -- _tDengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy -- _tDevelopment of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion -- _tPseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection -- _tCell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging -- _tDevelopment and Application of Dengue Virus Reverse Genetic Systems -- _tDENV4 Renilla Luciferase Reporter Replicons -- _tTargeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination -- _tIdentification of Dengue-Specific Human Antibody Fragments Using Phage Display -- _tNext Generation Whole Genome Sequencing of Dengue Virus -- _tMPGAfold in Dengue Secondary Structure Prediction -- _tInsights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE -- _tUse of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 -- _tIdentification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry -- _tAnalysis of Affinity of Dengue Virus Protein Interaction Using BIAcore -- _tFunctional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation -- _tInvestigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import -- _tSmall Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening -- _tConstruction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors -- _tConstruction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase -- _tAnimal Models in Dengue -- _tEvaluation of Dengue Antiviral Candidates In Vivo in Mouse Model. |
| 520 | _aInfection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research. | ||
| 588 | 0 | _aOnline resource; title from PDF title page (SpringerLink, viewed April 21, 2014). | |
| 650 | 0 |
_aDengue _vLaboratory manuals. _989563 |
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| 650 | 0 |
_aVirology _xTechnique. _983943 |
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| 650 | 0 |
_aVirology _vLaboratory manuals. _989564 |
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| 650 | 0 |
_aDengue viruses. _9669518 |
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| 650 | 1 | 2 |
_aDengue _xvirology _989565 |
| 650 | 1 | 2 |
_aDengue Virus _989566 |
| 650 | 6 |
_aDengue _vManuels de laboratoire. _9970812 |
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| 650 | 6 |
_aVirologie _xTechnique. _9970813 |
|
| 650 | 6 |
_aVirologie _vManuels de laboratoire. _9970814 |
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| 650 | 6 |
_aVirus de la dengue. _9970815 |
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| 650 | 7 |
_aDengue viruses _2fast _9669518 |
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| 650 | 7 |
_aDengue _2fast _989567 |
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| 650 | 7 |
_aVirology _2fast _981481 |
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| 650 | 7 |
_aVirology _xTechnique _2fast _983943 |
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| 655 | 2 | _aLaboratory Manual | |
| 655 | 7 |
_aLaboratory manuals _2fast |
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| 655 | 7 |
_aLaboratory manuals. _2lcgft |
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| 655 | 7 |
_aManuels de laboratoire. _2rvmgf _9966128 |
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| 700 | 1 |
_aPadmanabhan, Radhakrishnan, _eeditor. _989569 |
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| 700 | 1 |
_aVasudevan, Subhash, _eeditor. _989570 |
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| 776 | 0 | 8 |
_iPrint version: _tDengue. _dNew York, NY : Humana Press, [2014] _z9781493903474 _w(DLC) 2014933052 _w(OCoLC)881453825 |
| 830 | 0 |
_aMethods in molecular biology (Clifton, N.J.) ; _vv. 1138. _x1064-3745 |
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| 856 | 4 | 0 | _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-4939-0348-1 |
| 938 |
_aInternet Archive _bINAR _nisbn_9781493903474 |
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