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020 _a9780121820831
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049 _aMAIN
245 0 0 _aGuide to protein purification /
_cedited by Murray P. Deutscher.
260 _aSan Diego :
_bAcademic Press,
_c©1990.
300 _a1 online resource (xxix, 894 pages) :
_billustrations
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
490 1 _aMethods in enzymology,
_x0076-6879 ;
_vv. 182
504 _aIncludes bibliographical references and indexes.
588 0 _aPrint version record.
520 _aGuide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for protein and enzyme purification * Personal perspectives on enzyme purification by eminent researchers Among the Topics Covered * General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.
505 0 _aWhy purify enzymes / Arthur Kornberg -- Strategies and considerations for protein purifications / Stuart Linn -- Setting up a laboratory / Murray P. Deutscher -- Buffers: Principles and practice / Vincent S. Stoll, John S. Blanchard -- Measurement of enzyme activity / Edward F. Rossomando -- Quantitation of protein / Christa M. Stoscheck -- Concentration of proteins and removal of solutes / Thomas Pohl -- Maintaining protein stability / Murray P. Deutscher -- Overproduction of proteins in Escherichia coli: Vectors, hosts, and strategies / Asis Das -- Overexpression of proteins in eukaryotes / Margaret K. Bradley -- Secretion of heterologous proteins in Escherichia coli / I. Barry Holland, Brendan Kenny, Boris Steipe, Andreas Plückthun -- Preparation of extracts from prokaryotes / Millard Cull, Charles S. McHenry -- Preparation of extracts from yeast / S. Michal Jazwinski -- Preparation of Extracts from Plants / Peter Gegenheimer -- Preparation of extracts from higher eukaryotes / John David Dignam -- Isolation of subcellular organelles / Brian Storrie, Edward A Madden -- Preparation of membrane fractions / Juris Ozols -- Detergents: An overview / Judith M. Neugebauer -- Solubilization of native membrane proteins / Leonard M. Hjelmeland -- Solubilization of protein aggregates / Fiona A.O. Marston, Donna L. Hartley -- Removal of detergents from membrane proteins / Leonard M. Hjelmeland -- Precipitation techniques / Sasha Englard, Sam Seifter -- Precipitation of proteins with polyethylene glycol / Kenneth C. Ingham -- Ion-exchange chromatography / Edward F. Rossomando -- Gel filtration / Earle Stellwagen -- Protein chromatography on hydroxyapatite columns / Marina J. Gorbunoff -- Hydrophobic chromatography / Robert M. Kennedy -- Chromatography on immobilized reactive dyes / Earle Stellwagen -- Affinity chromatography: General methods / Steven Ostrove -- Affinity chromatography: Specialized techniques / Steven Ostrove, Shelly Weiss -- Chromatofocusing / Lallan Giri -- High-performance liquid chromatography: Effective protein purification by various chromatographic modes / Roman M. Chicz, Fred E. Regnier -- One-dimensional gel electrophoresis / David E. Garfin -- Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura, Therese M. Timmons -- Isoelectric focusing / David E. Garfin -- Gel-staining techniques / Carl R. Merril -- Elution of protein from gels / Michael G. Harrington -- Purification of membrane proteins / Thomas C. Thomas, Mark G. McNamee -- Purification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg, Stephen C. Francesconi, Chris Civalier, Scott S. Walker -- Purification of glycoproteins / Craig Gerard -- Purification of multienzyme complexes / Paul A. Srere, Christopher K. Mathews -- Determination of purity / David G. Rhodes, Thomas M. Laue -- Determination of size, molecular weight, and presence of subunits / Thomas M. Laue, David G. Rhodes -- Amino acid analysis / Juris Ozols -- Limited N-terminal sequence analysis / Paul Matsudaira -- Peptide mapping / Ralph C. Judd -- Analysis for protein modifications and nonprotein cofactors / Sam Seifter, Sasha Englard -- Protein crystallization / David Ollis, Stephen White -- Preparation of polyclonal antibodies / Bonnie S. Dunbar, Eric D. Schwoebel -- Preparation of monoclonal antibodies / Bonnie S. Dunbar, Sheri M. Skinner -- Protein blotting and immunodetection / Therese M. Timmons, Bonnie S. Dunbar -- Immunoprecipitation of proteins / Gary L. Firestone, Sandra D. Winguth -- Immunoassays / Charles W. Parker -- Radiolabeling of proteins / Charles W. Parker -- Using purified protein to clone its gene / John M. Wozney -- Computer analysis of protein structure / Patrick Argos -- Rethinking your purification procedure / Murray P. Deutscher -- The private life of DNA polymerase I / Arthur Kornberg -- Purification and crystallization of transaldolase from Candida utilis / B.L. Horecker, O. Tsolas -- Discovery of glutamine synthetase cascade / Earl R. Stadtman -- Discovery, resolution, purification, and function of elongation factors / Kivie Moldave.
506 _3Use copy
_fRestrictions unspecified
_2star
_5MiAaHDL
533 _aElectronic reproduction.
_b[Place of publication not identified] :
_cHathiTrust Digital Library,
_d2011.
_5MiAaHDL
538 _aMaster and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
_uhttp://purl.oclc.org/DLF/benchrepro0212
_5MiAaHDL
583 1 _adigitized
_c2011
_hHathiTrust Digital Library
_lcommitted to preserve
_2pda
_5MiAaHDL
546 _aEnglish.
650 0 _aProteins
_xPurification.
_981329
650 0 _aProteins.
_979920
650 2 _aProteins
_xisolation & purification
_981327
650 6 _aProtéines
_xPurification.
_9128823
650 6 _aProtéines.
_990146
650 7 _aprotein.
_2aat
_987594
650 7 _aProteins
_xPurification
_2fast
_981329
650 7 _aProteine
_2gnd
_94220
650 7 _aReinigung
_2gnd
_9128824
650 7 _aReinigungsverfahren
_2gnd
_9128825
650 1 7 _aEiwitten.
_2gtt
_980336
650 1 7 _aEnzymen.
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650 1 7 _aZuiveringstechnieken.
_2gtt
_984879
650 1 7 _aLaboratoriumonderzoek.
_2gtt
_980323
653 0 _aProteine
_aReinigungsverfahren
653 0 _aProteine
_aReinigung
655 4 _aElectronic books.
_9396
700 1 _aDeutscher, Murray P.,
_eeditor.
_1https://id.oclc.org/worldcat/entity/E39PCjBXT3qyvgfBx3QbB83KHP
_9102493
776 0 8 _iPrint version:
_tGuide to protein purification.
_dSan Diego : Academic Press, ©1990
_z0121820831
_w(OCoLC)22543482
830 0 _aMethods in enzymology ;
_vv. 182.
_x0076-6879
_997896
856 4 0 _uhttps://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/00766879/182
936 _aBATCHLOAD
938 _aYBP Library Services
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