Bioluminescence methods and protocols /
Bioluminescence methods and protocols /
edited by Robert A. LaRossa.
- Totowa, N.J. : Humana Press, 1998.
- 1 online resource (x, 310 pages) : illustrations
- Methods in molecular biology ; v. 102 .
- Methods in molecular biology (Clifton, N.J.) ; v. 102. .
Includes bibliographical references and index.
Improvements in the application of firefly luciferase assays / Sharon R. Ford, Franklin R. Leach -- Visualization of bioluminescence / Amy Cheng Vollmer -- Microscopic imagery of mammalian cells expressing an enhanced green fluorescent protein gene / Steven R. Kain, Guohong Zhang, Vanessa Gurtu, Paul A. Kitts -- Luminometric measurement of malate and glucose-6-phosphate in mammalian tissue / Eberhard Jüngling, Helmut Kammermeier, Yvan Fischer -- Bioluminescent assay of the guanylates / Sharon R. Ford, Franklin R. Leach -- Bioluminescent assay of the adenylate energy charge / Sharon R. Ford, Franklin R. Leach -- Photorhabdus luminescens luxCDABE promoter probe vectors / Tina K. Dyk, Reinhardt A. Rosson -- Insertion of promoter Region::luxCDABE fusions into the Escherichia coli chromosome / A. Elsemore David -- Probing for promoters with luciferase-transposons / David C. Alexander, Michael S. DuBow -- Cryopreservation and reawakening / L. Winona Wagner, Tina K. Dyk -- Luciferase renaturation assays of chaperones and chaperone antagonists / Vanitha Thulasiraman, Robert L. Matts -- Genotoxic sensors / Amy Cheng Vollmer -- Stress detection using bioluminescent reporters of the heat-shock response / Tina K. Dyk -- Real-time reporter of protein synthesis inhibition / Matti Korpela, Marko Virta, Matti Karp -- Luminescence-based cell viability testing / Ian A. Cree -- Phagocyte chemiluminescence / Ian A. Cree -- Detection of oxidants using lux fusions to oxidative stress promoters / Shimshon Belkin -- Luciferase-based measurement of water contaminants / Michael A. Costanzo, Julie Guzzo, Michael S. DuBow -- Bioluminescence-based metal detectors / Marko Virta, Sisko Tauriainen, Matti Karp -- Luminescence facilitated detection of bioavailable mercury in natural waters / Tamar Barkay, Ralph R. Turner, Lasse D. Rasmussen, Carol A. Kelly, John W. Rudd -- A panel of stress-responsive luminous bacteria for monitoring wastewater toxicity / Shimshon Belkin -- Organic contaminant detection and biodegradation characteristics / Robert S. Burlage -- Detection of firefly luciferase-tagged bacteria in environmental samples / Annelie Möller, Janet K. Jansson -- Monitoring of GFP-tagged bacterial cells / Riccardo Tombolini, Janet K. Jansson.
Comprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included. Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.
9781592595778 1592595774
Bioluminescence assay.
Luminescent Measurements
Biosensing Techniques
Tests de bioluminescence.
Bioluminescence assay
bioluminescentie bioluminescence luminescentie luminescence methodologie methodology biologische technieken biological techniques biochemie biochemistry moleculaire biologie molecular biology protocollen protocols Biological Techniques Biologische technieken
Laboratory Manual
Fulltext.
Internet Resource.
Laboratory manuals.
Manuels de laboratoire.
QP519.9.B55 / B536 1998
572/.36
QH506.M45 / v.102
W1 / ME9616J v.102 1998 QH 641 / B6154 1998
Includes bibliographical references and index.
Improvements in the application of firefly luciferase assays / Sharon R. Ford, Franklin R. Leach -- Visualization of bioluminescence / Amy Cheng Vollmer -- Microscopic imagery of mammalian cells expressing an enhanced green fluorescent protein gene / Steven R. Kain, Guohong Zhang, Vanessa Gurtu, Paul A. Kitts -- Luminometric measurement of malate and glucose-6-phosphate in mammalian tissue / Eberhard Jüngling, Helmut Kammermeier, Yvan Fischer -- Bioluminescent assay of the guanylates / Sharon R. Ford, Franklin R. Leach -- Bioluminescent assay of the adenylate energy charge / Sharon R. Ford, Franklin R. Leach -- Photorhabdus luminescens luxCDABE promoter probe vectors / Tina K. Dyk, Reinhardt A. Rosson -- Insertion of promoter Region::luxCDABE fusions into the Escherichia coli chromosome / A. Elsemore David -- Probing for promoters with luciferase-transposons / David C. Alexander, Michael S. DuBow -- Cryopreservation and reawakening / L. Winona Wagner, Tina K. Dyk -- Luciferase renaturation assays of chaperones and chaperone antagonists / Vanitha Thulasiraman, Robert L. Matts -- Genotoxic sensors / Amy Cheng Vollmer -- Stress detection using bioluminescent reporters of the heat-shock response / Tina K. Dyk -- Real-time reporter of protein synthesis inhibition / Matti Korpela, Marko Virta, Matti Karp -- Luminescence-based cell viability testing / Ian A. Cree -- Phagocyte chemiluminescence / Ian A. Cree -- Detection of oxidants using lux fusions to oxidative stress promoters / Shimshon Belkin -- Luciferase-based measurement of water contaminants / Michael A. Costanzo, Julie Guzzo, Michael S. DuBow -- Bioluminescence-based metal detectors / Marko Virta, Sisko Tauriainen, Matti Karp -- Luminescence facilitated detection of bioavailable mercury in natural waters / Tamar Barkay, Ralph R. Turner, Lasse D. Rasmussen, Carol A. Kelly, John W. Rudd -- A panel of stress-responsive luminous bacteria for monitoring wastewater toxicity / Shimshon Belkin -- Organic contaminant detection and biodegradation characteristics / Robert S. Burlage -- Detection of firefly luciferase-tagged bacteria in environmental samples / Annelie Möller, Janet K. Jansson -- Monitoring of GFP-tagged bacterial cells / Riccardo Tombolini, Janet K. Jansson.
Comprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included. Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.
9781592595778 1592595774
Bioluminescence assay.
Luminescent Measurements
Biosensing Techniques
Tests de bioluminescence.
Bioluminescence assay
bioluminescentie bioluminescence luminescentie luminescence methodologie methodology biologische technieken biological techniques biochemie biochemistry moleculaire biologie molecular biology protocollen protocols Biological Techniques Biologische technieken
Laboratory Manual
Fulltext.
Internet Resource.
Laboratory manuals.
Manuels de laboratoire.
QP519.9.B55 / B536 1998
572/.36
QH506.M45 / v.102
W1 / ME9616J v.102 1998 QH 641 / B6154 1998