Bioluminescence methods and protocols / edited by Robert A. LaRossa.

Includes bibliographical references and index.

Improvements in the application of firefly luciferase assays / Sharon R. Ford, Franklin R. Leach -- Visualization of bioluminescence / Amy Cheng Vollmer -- Microscopic imagery of mammalian cells expressing an enhanced green fluorescent protein gene / Steven R. Kain, Guohong Zhang, Vanessa Gurtu, Paul A. Kitts -- Luminometric measurement of malate and glucose-6-phosphate in mammalian tissue / Eberhard Jüngling, Helmut Kammermeier, Yvan Fischer -- Bioluminescent assay of the guanylates / Sharon R. Ford, Franklin R. Leach -- Bioluminescent assay of the adenylate energy charge / Sharon R. Ford, Franklin R. Leach -- Photorhabdus luminescens luxCDABE promoter probe vectors / Tina K. Dyk, Reinhardt A. Rosson -- Insertion of promoter Region::luxCDABE fusions into the Escherichia coli chromosome / A. Elsemore David -- Probing for promoters with luciferase-transposons / David C. Alexander, Michael S. DuBow -- Cryopreservation and reawakening / L. Winona Wagner, Tina K. Dyk -- Luciferase renaturation assays of chaperones and chaperone antagonists / Vanitha Thulasiraman, Robert L. Matts -- Genotoxic sensors / Amy Cheng Vollmer -- Stress detection using bioluminescent reporters of the heat-shock response / Tina K. Dyk -- Real-time reporter of protein synthesis inhibition / Matti Korpela, Marko Virta, Matti Karp -- Luminescence-based cell viability testing / Ian A. Cree -- Phagocyte chemiluminescence / Ian A. Cree -- Detection of oxidants using lux fusions to oxidative stress promoters / Shimshon Belkin -- Luciferase-based measurement of water contaminants / Michael A. Costanzo, Julie Guzzo, Michael S. DuBow -- Bioluminescence-based metal detectors / Marko Virta, Sisko Tauriainen, Matti Karp -- Luminescence facilitated detection of bioavailable mercury in natural waters / Tamar Barkay, Ralph R. Turner, Lasse D. Rasmussen, Carol A. Kelly, John W. Rudd -- A panel of stress-responsive luminous bacteria for monitoring wastewater toxicity / Shimshon Belkin -- Organic contaminant detection and biodegradation characteristics / Robert S. Burlage -- Detection of firefly luciferase-tagged bacteria in environmental samples / Annelie Möller, Janet K. Jansson -- Monitoring of GFP-tagged bacterial cells / Riccardo Tombolini, Janet K. Jansson.

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Comprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included. Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.