RNA scaffolds : methods and protocols /
RNA scaffolds : methods and protocols /
edited by Luc Ponchon.
- 1 online resource (xiii, 231 pages) : illustrations (some color)
- Methods in Molecular Biology, 1316 1940-6029 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 1316. .
Includes bibliographical references and index.
Method to predict the 3D structure of an RNA scaffold / Post-crystallization improvement of RNA crystal diffraction quality / Expression and purification of RNA-protein complexes in Escherichia coli / Production of homogeneous recombinant RNA using a tRNA scaffold and hammerhead ribozymes / In vivo production of small recombinant RNAs embedded in a 5S rRNA-derived protective scaffold / Detection of RNA-protein interactions using tethered RNA affinity capture / Universal method for labeling native RNA in live bacterial cells / Live cell imaging using riboswitch-spinach tRNA fusions as metabolite-sensing fluorescent biosensors / RNA scaffold : designed to co-localize enzymes / Artificial ligase ribozymes isolated by a "Design and Selection" strategy / Engineering aptazyme switches for conditional gene expression in mammalian cells utilizing an in vivo screening approach / Aptazyme-based riboswitches and logic gates in mammalian cells / Design and characterization of topological small RNAs / Folding RNA-protein complex into designed nanostructures / Simple method for constructing RNA triangle, square, pentagon by tuning interior RNA 3WJ angle from 60° to 90° or 108° / RNA-mediated CdS-based nanostructures / Effective method for specific gene silencing in Escherichia coli using artificial small RNA / Xiaojun Xu and Shi-Jie Chen -- Jinwei Zhang and Adrian R. Ferré-D'Amaré -- Margueritte El Khouri [and six others] -- Frank H.T. Nelissen, Hans A. Heus, and Sybren S. Wijmenga -- Victor G. Stepanov and George E. Fox -- Hidekazu Iioka and Ian G. Macara -- Irina Smolina and Natalia Broude -- Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond -- ZJU_China Team (iGEM 2012) and Ming Chen -- Shigeyoshi Matsumura and Yoshiya Ikawa -- Charlotte Rehm, Benedikt Klauser, and Jörg S. Hartig -- Yoko Nomura and Yohei Yokobayashi -- Jack Hassall [and four others] -- Tomonori Shibata [and four others] -- Emil F. Khisamutdinov [and five others] -- Vinit Kumar and Anil Kumar -- Geunu Bak [and four others].
This volume provides techniques on recent developments that use RNA scaffolds as molecular tools. RNA Scaffolds: Methods and Protocols guides readers through methods on various domains as molecular biology, nanotechnologies, and structural biology. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Scaffolds: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.
9781493927302 1493927302 1493927299 9781493927296
10.1007/978-1-4939-2730-2 doi
com.springer.onix.9781493927302 Springer Nature
2015937809
GBB8L1572 bnb
101665802 DNLM 019097978 Uk
RNA--Laboratory manuals.
Synthetic biology--Laboratory manuals.
RNA.
RNA
Cell Nucleus Structures
ARN--Manuels de laboratoire.
Biologie synthétique--Manuels de laboratoire.
ARN.
Biochemistry.
Science--Life Sciences--Biochemistry.
RNA
Synthetic biology
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QP623
572.8/8
W1 / ME9616J v.1316 2015 QU 25
Includes bibliographical references and index.
Method to predict the 3D structure of an RNA scaffold / Post-crystallization improvement of RNA crystal diffraction quality / Expression and purification of RNA-protein complexes in Escherichia coli / Production of homogeneous recombinant RNA using a tRNA scaffold and hammerhead ribozymes / In vivo production of small recombinant RNAs embedded in a 5S rRNA-derived protective scaffold / Detection of RNA-protein interactions using tethered RNA affinity capture / Universal method for labeling native RNA in live bacterial cells / Live cell imaging using riboswitch-spinach tRNA fusions as metabolite-sensing fluorescent biosensors / RNA scaffold : designed to co-localize enzymes / Artificial ligase ribozymes isolated by a "Design and Selection" strategy / Engineering aptazyme switches for conditional gene expression in mammalian cells utilizing an in vivo screening approach / Aptazyme-based riboswitches and logic gates in mammalian cells / Design and characterization of topological small RNAs / Folding RNA-protein complex into designed nanostructures / Simple method for constructing RNA triangle, square, pentagon by tuning interior RNA 3WJ angle from 60° to 90° or 108° / RNA-mediated CdS-based nanostructures / Effective method for specific gene silencing in Escherichia coli using artificial small RNA / Xiaojun Xu and Shi-Jie Chen -- Jinwei Zhang and Adrian R. Ferré-D'Amaré -- Margueritte El Khouri [and six others] -- Frank H.T. Nelissen, Hans A. Heus, and Sybren S. Wijmenga -- Victor G. Stepanov and George E. Fox -- Hidekazu Iioka and Ian G. Macara -- Irina Smolina and Natalia Broude -- Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond -- ZJU_China Team (iGEM 2012) and Ming Chen -- Shigeyoshi Matsumura and Yoshiya Ikawa -- Charlotte Rehm, Benedikt Klauser, and Jörg S. Hartig -- Yoko Nomura and Yohei Yokobayashi -- Jack Hassall [and four others] -- Tomonori Shibata [and four others] -- Emil F. Khisamutdinov [and five others] -- Vinit Kumar and Anil Kumar -- Geunu Bak [and four others].
This volume provides techniques on recent developments that use RNA scaffolds as molecular tools. RNA Scaffolds: Methods and Protocols guides readers through methods on various domains as molecular biology, nanotechnologies, and structural biology. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Scaffolds: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.
9781493927302 1493927302 1493927299 9781493927296
10.1007/978-1-4939-2730-2 doi
com.springer.onix.9781493927302 Springer Nature
2015937809
GBB8L1572 bnb
101665802 DNLM 019097978 Uk
RNA--Laboratory manuals.
Synthetic biology--Laboratory manuals.
RNA.
RNA
Cell Nucleus Structures
ARN--Manuels de laboratoire.
Biologie synthétique--Manuels de laboratoire.
ARN.
Biochemistry.
Science--Life Sciences--Biochemistry.
RNA
Synthetic biology
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QP623
572.8/8
W1 / ME9616J v.1316 2015 QU 25