RNA scaffolds : methods and protocols / edited by Luc Ponchon.

Includes bibliographical references and index.

Method to predict the 3D structure of an RNA scaffold / Xiaojun Xu and Shi-Jie Chen -- Post-crystallization improvement of RNA crystal diffraction quality / Jinwei Zhang and Adrian R. Ferré-D'Amaré -- Expression and purification of RNA-protein complexes in Escherichia coli / Margueritte El Khouri [and six others] -- Production of homogeneous recombinant RNA using a tRNA scaffold and hammerhead ribozymes / Frank H.T. Nelissen, Hans A. Heus, and Sybren S. Wijmenga -- In vivo production of small recombinant RNAs embedded in a 5S rRNA-derived protective scaffold / Victor G. Stepanov and George E. Fox -- Detection of RNA-protein interactions using tethered RNA affinity capture / Hidekazu Iioka and Ian G. Macara -- Universal method for labeling native RNA in live bacterial cells / Irina Smolina and Natalia Broude -- Live cell imaging using riboswitch-spinach tRNA fusions as metabolite-sensing fluorescent biosensors / Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond -- RNA scaffold : designed to co-localize enzymes / ZJU_China Team (iGEM 2012) and Ming Chen -- Artificial ligase ribozymes isolated by a "Design and Selection" strategy / Shigeyoshi Matsumura and Yoshiya Ikawa -- Engineering aptazyme switches for conditional gene expression in mammalian cells utilizing an in vivo screening approach / Charlotte Rehm, Benedikt Klauser, and Jörg S. Hartig -- Aptazyme-based riboswitches and logic gates in mammalian cells / Yoko Nomura and Yohei Yokobayashi -- Design and characterization of topological small RNAs / Jack Hassall [and four others] -- Folding RNA-protein complex into designed nanostructures / Tomonori Shibata [and four others] -- Simple method for constructing RNA triangle, square, pentagon by tuning interior RNA 3WJ angle from 60° to 90° or 108° / Emil F. Khisamutdinov [and five others] -- RNA-mediated CdS-based nanostructures / Vinit Kumar and Anil Kumar -- Effective method for specific gene silencing in Escherichia coli using artificial small RNA / Geunu Bak [and four others].

Online resource; title from PDF title page (SpringerLink, viewed May 15, 2015).

This volume provides techniques on recent developments that use RNA scaffolds as molecular tools. RNA Scaffolds: Methods and Protocols guides readers through methods on various domains as molecular biology, nanotechnologies, and structural biology. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Scaffolds: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.