Breast cancer : methods and protocols /
Breast cancer : methods and protocols /
edited by Jian Cao.
- 1 online resource (xii, 332 pages) : illustrations (some color)
- Methods in Molecular Biology, v. 1406 1064-3745 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 1406. .
Includes index.
Includes bibliographical references and index.
Basic histopathological methods and breast lesion lypes for research / Nengtai Ouyang and Lin Wang -- Clinical applications for immunohistochemistry of breast lesions / Kester Haye, Rajarsi Gupta, Christopher Metter, and Jingxuan Liu -- Immunohistochemistry for triple-negative breast cancer / Kalnisha Naidoo and Sarah E. Pinder -- In situ hybridzation of breast cancer markers / Li Min and Chengchao Shou -- Evaluation of human epidermal growth factor receptor 2 (HER2) gene status in human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue specimens by fluorescence in situ hybridization (FISH) / Harry C. Hwang and Allen M. Gown. Quantification of mRNA levels using real-time polymerase chain reaction (PCR) / Yiyi Li, Kai Wang, Longhua Chen, Xiaoxia Zhu, and Jie Zhou -- Detection of miRNA in cultured cells or xenograft tissues of breast cancer / Martin Brown and Meiyun Fan -- Pyrosequencing analysis for breast cancer DNA methylome / Cem Kuscu and Canan Kuscu -- Vita-assay method of enrichment and identification of ciruclating cancer cells/circulating tumor cells (CTCs) / Shaun Tulley, Qiang Zhao, Huan Dong, Michael L. Pearl, and Wen-Tien Chen -- Breast cancer stem cell isolation / Xuanmao Jiao, Albert A. Rizvanov, Massimo Cristofanilli, Regina R. Miftakhova, and Richard G. Pestell. Cellular apoptosis assay of breast cancer / Yu Sun and Wei-Xing Zong -- Assessment of matrix metalloproteinases by gelatin zymography / Jillian Cathcart -- Assessment of synthetic matrix metalloproteinase inhibitors by fluorogenic substrate assay / Ty J. Lively, Dale B. Bosco, Zahraa I. Khamis, and Qing-Xiang Amy Sang -- Determination of breast cancer cell migratory ability / David Schmitt, Joel Andrews, and Ming Tan -- A novel collagen dot assay for monitoring cancer cell migration / Vincent M. Alford, Eric Roth, Qian Zhang, and Jian Cao -- Three-dimensional assay for studying breast cancer cell invasion / Nikki A. Evensen -- A combined phagocytosis and fluorescent substrate degradation assay to simultaneously assess cell migration and substrate degradation / Ashleigh Pulkoski-Gross -- Analysis of invadopodia formation in breast cancer cells / Ziqing Wang, Xiao Liang, Ming Cai, and Guangwei Du -- Patient-derived tumor xenograft models of breast cancer cells using genetically encoded biosensors / Maryia Lu, Li Wei Rachel Tay, Jingquan He, and Guangwei Du -- 3D in vitro model for breast cancer research using magnetic levitation and bioprinting method / Fransisca Leonard and Biana Godin. Methods for analyzing tumor angiogenesis in the chick chorioallantoic membrane model / Jaquelyn J. Ames, Terry Henderson, Lucy Liaw, and Peter C. Brooks -- Pharmacokinetics and pharmacodynamics in breast cancer animal models / Wei Wang, Subhasree Nag, and Ruiwen Zhang -- Intracellular delivery of fluorescently labeled polysaccharide nanoparticles to cultured breast cancer cells / Derek Rammelkamp, Weiyi Li, and Yizhi Meng -- Imaging matrix metalloporteinase activity implicated in breast cancer progression / Gregg B. Fields and Maciej J. Stawikowski.
Annotation This volume provides resources, ideas, and bench manuals for the study of breast cancer. The book is divided into five sections: methods used in clinical laboratory for diagnosis; methods used in both clinical and research laboratories for testing genetic alterations; methods used to study the behaviour of breast cancer cells; and methods used for mimicking human breast cancer in a living organism.
9781493934447 1493934449 1493934422 9781493934423
10.1007/978-1-4939-3444-7 doi 9781493934423
10.1007/978-1-4939-3444-7 00012511
GBB8M4803 bnb
101683141 DNLM 019140341 Uk
Breast--Cancer--Laboratory manuals.
Tumor markers.
Breast Neoplasms
Biomarkers, Tumor
Molecular Diagnostic Techniques
Sein--Cancer--Manuels de laboratoire.
Marqueurs tumoraux.
Oncology.
Medical--Oncology.
Tumor markers
Breast--Cancer
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
RC280.B8
616.99/449
W1 / ME98616J v.1406 2016 WP 25
Includes index.
Includes bibliographical references and index.
Basic histopathological methods and breast lesion lypes for research / Nengtai Ouyang and Lin Wang -- Clinical applications for immunohistochemistry of breast lesions / Kester Haye, Rajarsi Gupta, Christopher Metter, and Jingxuan Liu -- Immunohistochemistry for triple-negative breast cancer / Kalnisha Naidoo and Sarah E. Pinder -- In situ hybridzation of breast cancer markers / Li Min and Chengchao Shou -- Evaluation of human epidermal growth factor receptor 2 (HER2) gene status in human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue specimens by fluorescence in situ hybridization (FISH) / Harry C. Hwang and Allen M. Gown. Quantification of mRNA levels using real-time polymerase chain reaction (PCR) / Yiyi Li, Kai Wang, Longhua Chen, Xiaoxia Zhu, and Jie Zhou -- Detection of miRNA in cultured cells or xenograft tissues of breast cancer / Martin Brown and Meiyun Fan -- Pyrosequencing analysis for breast cancer DNA methylome / Cem Kuscu and Canan Kuscu -- Vita-assay method of enrichment and identification of ciruclating cancer cells/circulating tumor cells (CTCs) / Shaun Tulley, Qiang Zhao, Huan Dong, Michael L. Pearl, and Wen-Tien Chen -- Breast cancer stem cell isolation / Xuanmao Jiao, Albert A. Rizvanov, Massimo Cristofanilli, Regina R. Miftakhova, and Richard G. Pestell. Cellular apoptosis assay of breast cancer / Yu Sun and Wei-Xing Zong -- Assessment of matrix metalloproteinases by gelatin zymography / Jillian Cathcart -- Assessment of synthetic matrix metalloproteinase inhibitors by fluorogenic substrate assay / Ty J. Lively, Dale B. Bosco, Zahraa I. Khamis, and Qing-Xiang Amy Sang -- Determination of breast cancer cell migratory ability / David Schmitt, Joel Andrews, and Ming Tan -- A novel collagen dot assay for monitoring cancer cell migration / Vincent M. Alford, Eric Roth, Qian Zhang, and Jian Cao -- Three-dimensional assay for studying breast cancer cell invasion / Nikki A. Evensen -- A combined phagocytosis and fluorescent substrate degradation assay to simultaneously assess cell migration and substrate degradation / Ashleigh Pulkoski-Gross -- Analysis of invadopodia formation in breast cancer cells / Ziqing Wang, Xiao Liang, Ming Cai, and Guangwei Du -- Patient-derived tumor xenograft models of breast cancer cells using genetically encoded biosensors / Maryia Lu, Li Wei Rachel Tay, Jingquan He, and Guangwei Du -- 3D in vitro model for breast cancer research using magnetic levitation and bioprinting method / Fransisca Leonard and Biana Godin. Methods for analyzing tumor angiogenesis in the chick chorioallantoic membrane model / Jaquelyn J. Ames, Terry Henderson, Lucy Liaw, and Peter C. Brooks -- Pharmacokinetics and pharmacodynamics in breast cancer animal models / Wei Wang, Subhasree Nag, and Ruiwen Zhang -- Intracellular delivery of fluorescently labeled polysaccharide nanoparticles to cultured breast cancer cells / Derek Rammelkamp, Weiyi Li, and Yizhi Meng -- Imaging matrix metalloporteinase activity implicated in breast cancer progression / Gregg B. Fields and Maciej J. Stawikowski.
Annotation This volume provides resources, ideas, and bench manuals for the study of breast cancer. The book is divided into five sections: methods used in clinical laboratory for diagnosis; methods used in both clinical and research laboratories for testing genetic alterations; methods used to study the behaviour of breast cancer cells; and methods used for mimicking human breast cancer in a living organism.
9781493934447 1493934449 1493934422 9781493934423
10.1007/978-1-4939-3444-7 doi 9781493934423
10.1007/978-1-4939-3444-7 00012511
GBB8M4803 bnb
101683141 DNLM 019140341 Uk
Breast--Cancer--Laboratory manuals.
Tumor markers.
Breast Neoplasms
Biomarkers, Tumor
Molecular Diagnostic Techniques
Sein--Cancer--Manuels de laboratoire.
Marqueurs tumoraux.
Oncology.
Medical--Oncology.
Tumor markers
Breast--Cancer
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
RC280.B8
616.99/449
W1 / ME98616J v.1406 2016 WP 25