Breast cancer : methods and protocols / edited by Jian Cao.

Includes index.

Online resource; title from PDF title page (SpringerLink, viewed February 4, 2016).

Annotation This volume provides resources, ideas, and bench manuals for the study of breast cancer. The book is divided into five sections: methods used in clinical laboratory for diagnosis; methods used in both clinical and research laboratories for testing genetic alterations; methods used to study the behaviour of breast cancer cells; and methods used for mimicking human breast cancer in a living organism.

Basic histopathological methods and breast lesion lypes for research / Nengtai Ouyang and Lin Wang -- Clinical applications for immunohistochemistry of breast lesions / Kester Haye, Rajarsi Gupta, Christopher Metter, and Jingxuan Liu -- Immunohistochemistry for triple-negative breast cancer / Kalnisha Naidoo and Sarah E. Pinder -- In situ hybridzation of breast cancer markers / Li Min and Chengchao Shou -- Evaluation of human epidermal growth factor receptor 2 (HER2) gene status in human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue specimens by fluorescence in situ hybridization (FISH) / Harry C. Hwang and Allen M. Gown.

Quantification of mRNA levels using real-time polymerase chain reaction (PCR) / Yiyi Li, Kai Wang, Longhua Chen, Xiaoxia Zhu, and Jie Zhou -- Detection of miRNA in cultured cells or xenograft tissues of breast cancer / Martin Brown and Meiyun Fan -- Pyrosequencing analysis for breast cancer DNA methylome / Cem Kuscu and Canan Kuscu -- Vita-assay method of enrichment and identification of ciruclating cancer cells/circulating tumor cells (CTCs) / Shaun Tulley, Qiang Zhao, Huan Dong, Michael L. Pearl, and Wen-Tien Chen -- Breast cancer stem cell isolation / Xuanmao Jiao, Albert A. Rizvanov, Massimo Cristofanilli, Regina R. Miftakhova, and Richard G. Pestell.

Cellular apoptosis assay of breast cancer / Yu Sun and Wei-Xing Zong -- Assessment of matrix metalloproteinases by gelatin zymography / Jillian Cathcart -- Assessment of synthetic matrix metalloproteinase inhibitors by fluorogenic substrate assay / Ty J. Lively, Dale B. Bosco, Zahraa I. Khamis, and Qing-Xiang Amy Sang -- Determination of breast cancer cell migratory ability / David Schmitt, Joel Andrews, and Ming Tan -- A novel collagen dot assay for monitoring cancer cell migration / Vincent M. Alford, Eric Roth, Qian Zhang, and Jian Cao -- Three-dimensional assay for studying breast cancer cell invasion / Nikki A. Evensen -- A combined phagocytosis and fluorescent substrate degradation assay to simultaneously assess cell migration and substrate degradation / Ashleigh Pulkoski-Gross -- Analysis of invadopodia formation in breast cancer cells / Ziqing Wang, Xiao Liang, Ming Cai, and Guangwei Du -- Patient-derived tumor xenograft models of breast cancer cells using genetically encoded biosensors / Maryia Lu, Li Wei Rachel Tay, Jingquan He, and Guangwei Du -- 3D in vitro model for breast cancer research using magnetic levitation and bioprinting method / Fransisca Leonard and Biana Godin.

Methods for analyzing tumor angiogenesis in the chick chorioallantoic membrane model / Jaquelyn J. Ames, Terry Henderson, Lucy Liaw, and Peter C. Brooks -- Pharmacokinetics and pharmacodynamics in breast cancer animal models / Wei Wang, Subhasree Nag, and Ruiwen Zhang -- Intracellular delivery of fluorescently labeled polysaccharide nanoparticles to cultured breast cancer cells / Derek Rammelkamp, Weiyi Li, and Yizhi Meng -- Imaging matrix metalloporteinase activity implicated in breast cancer progression / Gregg B. Fields and Maciej J. Stawikowski.

Includes bibliographical references and index.