Chromosome and genomic engineering in plants : methods and protocols /
Chromosome and genomic engineering in plants : methods and protocols /
edited by Minoru Murata.
- 1 online resource (xi, 218 pages) : illustrations (some color)
- Methods in molecular biology, volume 1469 1064-3745 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 1469, .
Includes bibliographical references and index.
Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences -- Method for Biolistic Site-Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase -- Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking -- Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants -- One-Step Generation of Chromosomal Rearrangements in Rice -- Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana -- Gametocidal Systems for Dissecting Wheat Chromosomes -- CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases -- Targeted Mutagenesis in Rice Using and TALENs and the CRISPR/Cas9 System -- Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination -- Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured Cells -- Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes -- Image Analysis of DNA Fiber and Nucleus in Plants -- Detection of Transgenes on DNA Fibers -- Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging Systems -- Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes -- Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements.
This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a 'plant chromosome vector' system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination.
9781493949311 1493949314
10.1007/978-1-4939-4931-1 doi
com.springer.onix.9781493949311 Springer Nature
GBB8M4809 bnb
101696763 DNLM 019140347 Uk
Plant genetic engineering--Laboratory manuals.
Genetic engineering.
Transgenic plants.
Genetic Engineering
Plants, Genetically Modified
Génie génétique végétal--Manuels de laboratoire.
Génie génétique.
Plantes transgéniques.
Botany & plant sciences.
Science--Life Sciences--Botany.
Transgenic plants
Genetic engineering
Plant genetic engineering
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QK981.5
660.6/5
W1 / ME9616J v.1469 2016 QK 981.5
Includes bibliographical references and index.
Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences -- Method for Biolistic Site-Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase -- Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking -- Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants -- One-Step Generation of Chromosomal Rearrangements in Rice -- Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana -- Gametocidal Systems for Dissecting Wheat Chromosomes -- CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases -- Targeted Mutagenesis in Rice Using and TALENs and the CRISPR/Cas9 System -- Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination -- Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured Cells -- Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes -- Image Analysis of DNA Fiber and Nucleus in Plants -- Detection of Transgenes on DNA Fibers -- Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging Systems -- Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes -- Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements.
This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a 'plant chromosome vector' system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination.
9781493949311 1493949314
10.1007/978-1-4939-4931-1 doi
com.springer.onix.9781493949311 Springer Nature
GBB8M4809 bnb
101696763 DNLM 019140347 Uk
Plant genetic engineering--Laboratory manuals.
Genetic engineering.
Transgenic plants.
Genetic Engineering
Plants, Genetically Modified
Génie génétique végétal--Manuels de laboratoire.
Génie génétique.
Plantes transgéniques.
Botany & plant sciences.
Science--Life Sciences--Botany.
Transgenic plants
Genetic engineering
Plant genetic engineering
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QK981.5
660.6/5
W1 / ME9616J v.1469 2016 QK 981.5