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Chromosome and genomic engineering in plants : methods and protocols / edited by Minoru Murata.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1469,Publisher: New York, NY : Humana Press, 2016Description: 1 online resource (xi, 218 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781493949311
  • 1493949314
Subject(s): Genre/Form: Additional physical formats: Print version:: Chromosome and Genomic Engineering in Plants.DDC classification:
  • 660.6/5 23
LOC classification:
  • QK981.5
NLM classification:
  • W1
  • QK 981.5
Online resources:
Contents:
Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences -- Method for Biolistic Site-Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase -- Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking -- Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants -- One-Step Generation of Chromosomal Rearrangements in Rice -- Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana -- Gametocidal Systems for Dissecting Wheat Chromosomes -- CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases -- Targeted Mutagenesis in Rice Using and TALENs and the CRISPR/Cas9 System -- Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination -- Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured Cells -- Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes -- Image Analysis of DNA Fiber and Nucleus in Plants -- Detection of Transgenes on DNA Fibers -- Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging Systems -- Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes -- Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements.
Summary: This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a 'plant chromosome vector' system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination.
Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
eBook eBook e-Library eBook Protocols Available
Total holds: 0

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed September 1, 2016).

This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a 'plant chromosome vector' system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination.

Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences -- Method for Biolistic Site-Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase -- Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking -- Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants -- One-Step Generation of Chromosomal Rearrangements in Rice -- Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana -- Gametocidal Systems for Dissecting Wheat Chromosomes -- CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases -- Targeted Mutagenesis in Rice Using and TALENs and the CRISPR/Cas9 System -- Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination -- Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured Cells -- Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes -- Image Analysis of DNA Fiber and Nucleus in Plants -- Detection of Transgenes on DNA Fibers -- Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging Systems -- Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes -- Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements.

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