Vesicular transport. Part B /
Vesicular transport. Part B /
edited by Alan M. Tartakoff.
- San Diego : Academic Press, ©1989.
- 1 online resource (xx, 499 pages) : illustrations.
- Methods in cell biology ; v. 32 .
- Methods in cell biology ; v. 32. .
"Prepared under the auspices of the American Society for Cell Biology."
Includes bibliographical references and index.
Front Cover; Methods in Cell Biology: Vesicular Transport, Part B; Copyright Page; Contents; Contributors; Preface; PART I: MONITORING AND REGULATING THE PROGRESS OF TRANSPORT-SECRETORY PATH; Chapter 1. A Hitchhiker's Guide to Analysis of the Secretory Pathway in Yeast; I. Introduction; II. Yeast Strains and Growth Conditions; III. In Vivo Radiolabeling and Lysis of Yeast Cells; IV. Analysis of Intercompartmental Protein Transport; References; Chapter 2. Methods to Estimate the Polarized Distribution of Surface Antigens in Cultured Epithelial Cells; I. Polarity of Epithelial Cells II. Expression of Foreign Genes in Polarized Epithelial LinesIII. Estimation of Polarity of Epithelial Surface Molecules; IV. Conclusions; References; Chapter 3. Analysis of the Synthesis, Intracellular Sorting, and Function of Glycoproteins Using a Mammalian Cell Mutant with Reversible Glycosylation Defects; I. Introduction; II. Synthesis and Function of Protein-Linked Oligosaccharides; III. CHO Mutants with Defects in Oligosaccharide Biosynthesis; IV. Reversibility of the Glycosylation Defects in ldlD Cells; V. Glycoprotein Synthesis, Sorting, and Function Studied Using ldlD Cells VI. Care and Feeding of ldlD CellsReferences; Chapter 4. Using Temperature-Sensitive Murants of VSV to Study Membrane Protein Biogenesis; I. Introduction; II. Synchronizing the Transport of the ts045 G Protein; III. Prospects for Future Uses of Temperature-Sensitive Mutants; IV. Technical Procedures; References; Chapter 5. Enzymatic Approaches for Studying the Structure Synthesis, and Procesing of Glycoproteins; I. Introduction; II. Oligosaccharide-Cleaving Enzymes; III. Related Matters; References; Chapter 6. Separation and Analysis of Glycoprotein Oligosaccharides; I. Introduction II. Diversity of Sugar Chains in Animal Cell GlycoproteinsIII. Evidence Indicating that a Protein Is Glycosylated; IV. Radiolabeling of Sugar Chains; V. Enzymatic and Chemical Methods for Releasing Sugar Chains from Proteins; VI. Separation of Sugar Chains by Serial Lectin Affinity Chromatography; VII. Analysis of Metabolically Radiolabeled Sugar Chains; VIII. Conclusions; References; Chapter 7. Protein Folding and Intracellular Transport: Evaluation of Conformational Changes in Nascent Exocytotic Proteins; I. Introduction; II. Methods for Analysis of Protein Conformation III. Methods for Analysis of the Assembly of Nascent Proteins into OligomersIV. Conclusion; References; Chapter 8. Glycosaminoglycan Modifcations of Membrane Proteins; I. Introduction; II. Biochemical Characterization of a PG; III. Demonstration that the PG Core Protein Is Identical to the Given Unmodified Protein; IV. Determination of a Membrane Location of the PG; V. Conclusion; References; Chapter 9. Identification and Analysis of Glycoinositol Phospholipid Anchors in Membrane Proteins; I. Introduction; II. Cleavage of Anchored Proteins by Exogenous Phospholipase C
METHODS IN CELL BIOLOGY,VOLUME 32: VESICULAR TRANSPORT, PART B.
English.
9780080859309 0080859305 012564132X 9780125641326 1281513806 9781281513809 9786611513801 6611513809
Cytology--Technique.
Endocytosis.
Secretion.
Cytochemistry.
Biological transport.
Cell organelles.
Biological Transport
Endocytosis
Cytological Techniques
Bodily Secretions
Histocytochemistry
Cytologie--Technique.
Endocytose.
Sécrétion.
Cytochimie.
Transport biologique.
SCIENCE--Life Sciences--Cell Biology.
Biological transport
Cell organelles
Cytochemistry
Cytology--Technique
Endocytosis
Secretion
QH585 / .M47eb vol. 32, pt. B
571.6
"Prepared under the auspices of the American Society for Cell Biology."
Includes bibliographical references and index.
Front Cover; Methods in Cell Biology: Vesicular Transport, Part B; Copyright Page; Contents; Contributors; Preface; PART I: MONITORING AND REGULATING THE PROGRESS OF TRANSPORT-SECRETORY PATH; Chapter 1. A Hitchhiker's Guide to Analysis of the Secretory Pathway in Yeast; I. Introduction; II. Yeast Strains and Growth Conditions; III. In Vivo Radiolabeling and Lysis of Yeast Cells; IV. Analysis of Intercompartmental Protein Transport; References; Chapter 2. Methods to Estimate the Polarized Distribution of Surface Antigens in Cultured Epithelial Cells; I. Polarity of Epithelial Cells II. Expression of Foreign Genes in Polarized Epithelial LinesIII. Estimation of Polarity of Epithelial Surface Molecules; IV. Conclusions; References; Chapter 3. Analysis of the Synthesis, Intracellular Sorting, and Function of Glycoproteins Using a Mammalian Cell Mutant with Reversible Glycosylation Defects; I. Introduction; II. Synthesis and Function of Protein-Linked Oligosaccharides; III. CHO Mutants with Defects in Oligosaccharide Biosynthesis; IV. Reversibility of the Glycosylation Defects in ldlD Cells; V. Glycoprotein Synthesis, Sorting, and Function Studied Using ldlD Cells VI. Care and Feeding of ldlD CellsReferences; Chapter 4. Using Temperature-Sensitive Murants of VSV to Study Membrane Protein Biogenesis; I. Introduction; II. Synchronizing the Transport of the ts045 G Protein; III. Prospects for Future Uses of Temperature-Sensitive Mutants; IV. Technical Procedures; References; Chapter 5. Enzymatic Approaches for Studying the Structure Synthesis, and Procesing of Glycoproteins; I. Introduction; II. Oligosaccharide-Cleaving Enzymes; III. Related Matters; References; Chapter 6. Separation and Analysis of Glycoprotein Oligosaccharides; I. Introduction II. Diversity of Sugar Chains in Animal Cell GlycoproteinsIII. Evidence Indicating that a Protein Is Glycosylated; IV. Radiolabeling of Sugar Chains; V. Enzymatic and Chemical Methods for Releasing Sugar Chains from Proteins; VI. Separation of Sugar Chains by Serial Lectin Affinity Chromatography; VII. Analysis of Metabolically Radiolabeled Sugar Chains; VIII. Conclusions; References; Chapter 7. Protein Folding and Intracellular Transport: Evaluation of Conformational Changes in Nascent Exocytotic Proteins; I. Introduction; II. Methods for Analysis of Protein Conformation III. Methods for Analysis of the Assembly of Nascent Proteins into OligomersIV. Conclusion; References; Chapter 8. Glycosaminoglycan Modifcations of Membrane Proteins; I. Introduction; II. Biochemical Characterization of a PG; III. Demonstration that the PG Core Protein Is Identical to the Given Unmodified Protein; IV. Determination of a Membrane Location of the PG; V. Conclusion; References; Chapter 9. Identification and Analysis of Glycoinositol Phospholipid Anchors in Membrane Proteins; I. Introduction; II. Cleavage of Anchored Proteins by Exogenous Phospholipase C
METHODS IN CELL BIOLOGY,VOLUME 32: VESICULAR TRANSPORT, PART B.
English.
9780080859309 0080859305 012564132X 9780125641326 1281513806 9781281513809 9786611513801 6611513809
Cytology--Technique.
Endocytosis.
Secretion.
Cytochemistry.
Biological transport.
Cell organelles.
Biological Transport
Endocytosis
Cytological Techniques
Bodily Secretions
Histocytochemistry
Cytologie--Technique.
Endocytose.
Sécrétion.
Cytochimie.
Transport biologique.
SCIENCE--Life Sciences--Cell Biology.
Biological transport
Cell organelles
Cytochemistry
Cytology--Technique
Endocytosis
Secretion
QH585 / .M47eb vol. 32, pt. B
571.6