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Vesicular transport. Part B / edited by Alan M. Tartakoff.

Contributor(s): Material type: TextTextSeries: Methods in cell biology ; v. 32.Publication details: San Diego : Academic Press, ©1989.Description: 1 online resource (xx, 499 pages) : illustrationsContent type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9780080859309
  • 0080859305
  • 012564132X
  • 9780125641326
  • 1281513806
  • 9781281513809
  • 9786611513801
  • 6611513809
Subject(s): Additional physical formats: Print version:: Vesicular transport. Part B.DDC classification:
  • 571.6 22
LOC classification:
  • QH585 .M47eb vol. 32, pt. B
Online resources:
Contents:
Front Cover; Methods in Cell Biology: Vesicular Transport, Part B; Copyright Page; Contents; Contributors; Preface; PART I: MONITORING AND REGULATING THE PROGRESS OF TRANSPORT-SECRETORY PATH; Chapter 1. A Hitchhiker's Guide to Analysis of the Secretory Pathway in Yeast; I. Introduction; II. Yeast Strains and Growth Conditions; III. In Vivo Radiolabeling and Lysis of Yeast Cells; IV. Analysis of Intercompartmental Protein Transport; References; Chapter 2. Methods to Estimate the Polarized Distribution of Surface Antigens in Cultured Epithelial Cells; I. Polarity of Epithelial Cells
II. Expression of Foreign Genes in Polarized Epithelial LinesIII. Estimation of Polarity of Epithelial Surface Molecules; IV. Conclusions; References; Chapter 3. Analysis of the Synthesis, Intracellular Sorting, and Function of Glycoproteins Using a Mammalian Cell Mutant with Reversible Glycosylation Defects; I. Introduction; II. Synthesis and Function of Protein-Linked Oligosaccharides; III. CHO Mutants with Defects in Oligosaccharide Biosynthesis; IV. Reversibility of the Glycosylation Defects in ldlD Cells; V. Glycoprotein Synthesis, Sorting, and Function Studied Using ldlD Cells
VI. Care and Feeding of ldlD CellsReferences; Chapter 4. Using Temperature-Sensitive Murants of VSV to Study Membrane Protein Biogenesis; I. Introduction; II. Synchronizing the Transport of the ts045 G Protein; III. Prospects for Future Uses of Temperature-Sensitive Mutants; IV. Technical Procedures; References; Chapter 5. Enzymatic Approaches for Studying the Structure Synthesis, and Procesing of Glycoproteins; I. Introduction; II. Oligosaccharide-Cleaving Enzymes; III. Related Matters; References; Chapter 6. Separation and Analysis of Glycoprotein Oligosaccharides; I. Introduction
II. Diversity of Sugar Chains in Animal Cell GlycoproteinsIII. Evidence Indicating that a Protein Is Glycosylated; IV. Radiolabeling of Sugar Chains; V. Enzymatic and Chemical Methods for Releasing Sugar Chains from Proteins; VI. Separation of Sugar Chains by Serial Lectin Affinity Chromatography; VII. Analysis of Metabolically Radiolabeled Sugar Chains; VIII. Conclusions; References; Chapter 7. Protein Folding and Intracellular Transport: Evaluation of Conformational Changes in Nascent Exocytotic Proteins; I. Introduction; II. Methods for Analysis of Protein Conformation
III. Methods for Analysis of the Assembly of Nascent Proteins into OligomersIV. Conclusion; References; Chapter 8. Glycosaminoglycan Modifcations of Membrane Proteins; I. Introduction; II. Biochemical Characterization of a PG; III. Demonstration that the PG Core Protein Is Identical to the Given Unmodified Protein; IV. Determination of a Membrane Location of the PG; V. Conclusion; References; Chapter 9. Identification and Analysis of Glycoinositol Phospholipid Anchors in Membrane Proteins; I. Introduction; II. Cleavage of Anchored Proteins by Exogenous Phospholipase C
Summary: METHODS IN CELL BIOLOGY,VOLUME 32: VESICULAR TRANSPORT, PART B.
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"Prepared under the auspices of the American Society for Cell Biology."

Includes bibliographical references and index.

Print version record.

Front Cover; Methods in Cell Biology: Vesicular Transport, Part B; Copyright Page; Contents; Contributors; Preface; PART I: MONITORING AND REGULATING THE PROGRESS OF TRANSPORT-SECRETORY PATH; Chapter 1. A Hitchhiker's Guide to Analysis of the Secretory Pathway in Yeast; I. Introduction; II. Yeast Strains and Growth Conditions; III. In Vivo Radiolabeling and Lysis of Yeast Cells; IV. Analysis of Intercompartmental Protein Transport; References; Chapter 2. Methods to Estimate the Polarized Distribution of Surface Antigens in Cultured Epithelial Cells; I. Polarity of Epithelial Cells

II. Expression of Foreign Genes in Polarized Epithelial LinesIII. Estimation of Polarity of Epithelial Surface Molecules; IV. Conclusions; References; Chapter 3. Analysis of the Synthesis, Intracellular Sorting, and Function of Glycoproteins Using a Mammalian Cell Mutant with Reversible Glycosylation Defects; I. Introduction; II. Synthesis and Function of Protein-Linked Oligosaccharides; III. CHO Mutants with Defects in Oligosaccharide Biosynthesis; IV. Reversibility of the Glycosylation Defects in ldlD Cells; V. Glycoprotein Synthesis, Sorting, and Function Studied Using ldlD Cells

VI. Care and Feeding of ldlD CellsReferences; Chapter 4. Using Temperature-Sensitive Murants of VSV to Study Membrane Protein Biogenesis; I. Introduction; II. Synchronizing the Transport of the ts045 G Protein; III. Prospects for Future Uses of Temperature-Sensitive Mutants; IV. Technical Procedures; References; Chapter 5. Enzymatic Approaches for Studying the Structure Synthesis, and Procesing of Glycoproteins; I. Introduction; II. Oligosaccharide-Cleaving Enzymes; III. Related Matters; References; Chapter 6. Separation and Analysis of Glycoprotein Oligosaccharides; I. Introduction

II. Diversity of Sugar Chains in Animal Cell GlycoproteinsIII. Evidence Indicating that a Protein Is Glycosylated; IV. Radiolabeling of Sugar Chains; V. Enzymatic and Chemical Methods for Releasing Sugar Chains from Proteins; VI. Separation of Sugar Chains by Serial Lectin Affinity Chromatography; VII. Analysis of Metabolically Radiolabeled Sugar Chains; VIII. Conclusions; References; Chapter 7. Protein Folding and Intracellular Transport: Evaluation of Conformational Changes in Nascent Exocytotic Proteins; I. Introduction; II. Methods for Analysis of Protein Conformation

III. Methods for Analysis of the Assembly of Nascent Proteins into OligomersIV. Conclusion; References; Chapter 8. Glycosaminoglycan Modifcations of Membrane Proteins; I. Introduction; II. Biochemical Characterization of a PG; III. Demonstration that the PG Core Protein Is Identical to the Given Unmodified Protein; IV. Determination of a Membrane Location of the PG; V. Conclusion; References; Chapter 9. Identification and Analysis of Glycoinositol Phospholipid Anchors in Membrane Proteins; I. Introduction; II. Cleavage of Anchored Proteins by Exogenous Phospholipase C

METHODS IN CELL BIOLOGY,VOLUME 32: VESICULAR TRANSPORT, PART B.

English.

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