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Plant synthetic promoters : methods and protocols / edited by Reinhard Hehl.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1482.Publisher: New York, NY : Humana Press, 2016Description: 1 online resource (xi, 325 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781493963966
  • 1493963961
  • 1493963945
  • 9781493963942
Subject(s): Genre/Form: Additional physical formats: Print version:: Plant synthetic promoters.DDC classification:
  • 572.8/652 23
LOC classification:
  • QK981
NLM classification:
  • W1
  • QK 981
Online resources:
Contents:
What Have We Learned About Synthetic Promoter Construction? -- Quantitative Analysis of Cis-Regulatory Element Activity Using Synthetic Promoters in Transgenic Plants -- The Identification of Cis-Regulatory Sequence Motifs in Gene Promoters Based on SNP Information -- Quantitative Analysis of Protein-DNA Interaction by qDPI-ELISA -- Analyzing Synthetic Promoters Using Arabidopsis Protoplasts -- Selecting Hypomethylated Genomic Regions Using MRE-Seq -- Spatio-Temporal Imaging of Promoter Activity in Intact Plant Tissues -- Novel Synthetic Promoters from the Cestrum Yellow Leaf-Curling Virus -- Fast and Efficient Cloning of Cis-Regulatory Sequences for High Throughput Yeast One-Hybrid Analyses of Transcription Factors -- The Physcomitrella patens System for Transient Gene Expression Assays -- Analysis of Microbe-Associated Molecular Pattern-Responsive Synthetic Promoters with the Parsley Protoplast System -- A Framework for Discovering, Designing, and Testing MicroProteins to Regulate Synthetic Transcriptional Modules -- Simultaneous Analysis of Multiple Promoters: An Application of the PC-GW Binary Vector Series -- GenoCAD Plant Grammar to Design Plant Expression Vectors for Promoter Analysis -- Bioinformatic Identification of Conserved Cis-Sequences in Coregulated Genes -- In Silico Expression Analysis -- FootprintDB: Analysis of Plant Cis-Regulatory Elements, Transcription Factors, and Binding Interfaces -- RSAT::Plants: Motif Discovery Within Clusters of Upstream Sequences in Plant Genomes -- RSAT::Plants: Motif Discovery in ChIP-Seq Peaks of Plant Genomes.
Summary: This text assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis -regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented.
Holdings
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Includes index.

Online resource; title from PDF title page (SpringerLink, viewed September 1, 2016).

This text assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis -regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented.

What Have We Learned About Synthetic Promoter Construction? -- Quantitative Analysis of Cis-Regulatory Element Activity Using Synthetic Promoters in Transgenic Plants -- The Identification of Cis-Regulatory Sequence Motifs in Gene Promoters Based on SNP Information -- Quantitative Analysis of Protein-DNA Interaction by qDPI-ELISA -- Analyzing Synthetic Promoters Using Arabidopsis Protoplasts -- Selecting Hypomethylated Genomic Regions Using MRE-Seq -- Spatio-Temporal Imaging of Promoter Activity in Intact Plant Tissues -- Novel Synthetic Promoters from the Cestrum Yellow Leaf-Curling Virus -- Fast and Efficient Cloning of Cis-Regulatory Sequences for High Throughput Yeast One-Hybrid Analyses of Transcription Factors -- The Physcomitrella patens System for Transient Gene Expression Assays -- Analysis of Microbe-Associated Molecular Pattern-Responsive Synthetic Promoters with the Parsley Protoplast System -- A Framework for Discovering, Designing, and Testing MicroProteins to Regulate Synthetic Transcriptional Modules -- Simultaneous Analysis of Multiple Promoters: An Application of the PC-GW Binary Vector Series -- GenoCAD Plant Grammar to Design Plant Expression Vectors for Promoter Analysis -- Bioinformatic Identification of Conserved Cis-Sequences in Coregulated Genes -- In Silico Expression Analysis -- FootprintDB: Analysis of Plant Cis-Regulatory Elements, Transcription Factors, and Binding Interfaces -- RSAT::Plants: Motif Discovery Within Clusters of Upstream Sequences in Plant Genomes -- RSAT::Plants: Motif Discovery in ChIP-Seq Peaks of Plant Genomes.

Includes bibliographical references and index.

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